Growth characteristics of HCT116 xenografts lacking asparagine synthetase vary according to sex

Jun 17, 2024·
Oladimeji Aladelokun
,
Lingeng Lu
,
Jie Zheng
Hong Yan
Hong Yan
,
Abhishek Jain
Joanna Gibson
Joanna Gibson
Sajid A. Khan
Sajid A. Khan
Caroline H. Johnson
Caroline H. Johnson
· 1 min read

Background

Sex-related differences in colorectal (CRC) incidence and mortality are well-documented. However, the impact of sex on metabolic pathways that drive cancer growth is not well understood. High expression of asparagine synthetase (ASNS) is associated with inferior survival for female CRC patients only. Here, we used a CRISPR/Cas9 technology to generate HCT116 ASNS−/− and HCT 116 ASNS+/+ cancer cell lines. We examine the effects of ASNS deletion on tumor growth and the subsequent rewiring of metabolic pathways in male and female Rag2/IL2RG mice.

Publication

Human Genomics

Type

Journal Articles

Hong Yan
Authors
Research Assistant Professor
Joanna Gibson
Authors
Joanna Gibson
Associate Professor
Yale University
Sajid A. Khan
Authors
Sajid A. Khan
Associate Professor
Yale University
Caroline H. Johnson
Authors
Caroline H. Johnson
Associate Professor
Yale University